Invitrogen

CD90.2 (Thy-1.2) Monoclonal Antibody (30-H12), PE, eBioscience™

CD90.2 (Thy-1.2) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 4

CD90.2 (Thy-1.2) Antibody (12-0903-81) in Flow

Staining of C57Bl/6 splenocytes with Anti-Mouse CD3e APC (Product # 17-0031-82) and 0.06 µg of Rat IgG2b kappa Isotype Control PE (Product # 12-4031-82) (left) or 0.06 µg of Anti-Mouse CD90.2 (Thy-1.2) PE (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

12-0903-81

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.125 µg/test

View 18 publications 18 publications

Product Specifications

Published species

Human, Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

30-H12

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Wet ice

RRID

AB_465778

Product Specific Information

Description: The 30-H12 monoclonal antibody reacts with mouse CD90.2, also known as Thy-1.2, a GPI-linked membrane molecule. CD90.2 is expressed by mouse thymocytes and mature T cells as well as neurons in CD90.2-expressing mouse strains. These strains include BALB/c, CBA, C3H, C57BL/6, C58/J, SJL and others. Cells from CD90.1-expressing strains including PL and AKR do not stain with 30-H12. CD90 is involved in regulation of adhesion and signal transduction by T cells.

Applications Reported: The 30-H12 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 30-H12 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD90.2 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. CD90.2 is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts. Thy 1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. CD90.2 is an 18 kDa plasma membrane antigen expressed by hematopoietic cells and neurons in all species studied, highly expressed in connective tissue, various fibroblast and stromal cell lines and lastly, by tumor endothelial cell lines. In the mouse, CD90.2 is expressed on all thymocytes and peripheral T lymphocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Bcl-xL mutant promotes cartilage differentiation of BMSCs by upregulating TGF-β/BMP expression levels.

Experimental and therapeutic medicine

Xiao K,Yang L,Xie W,Gao X,Huang R,Xie M

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 12-0903-82) in Flow Cytometry

Thu Jul 01 00:00:00 EDT 2021

Adipose-derived stem cells therapy effectively attenuates PM_2.5-induced lung injury.

Stem cell research & therapy

Gao J,Yuan J,Liu Q,Wang Y,Wang H,Chen Y,Ding W,Ji G,Lu Z

12-0903-82 was used in Flow Cytometry to suggest that ADSC transplantation may be a potential therapeutic approach for severe air pollution-associated diseases.

Sat Jun 19 00:00:00 EDT 2021

Mechanisms of interleukin 4 mediated increase in efficacy of vaccines against opioid use disorders.

NPJ vaccines

Crouse B,Robinson C,Huseby Kelcher A,Laudenbach M,Abrahante JE,Pravetoni M

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 12-0903-82) in Flow Cytometry

Thu Oct 29 00:00:00 EDT 2020

Nestin-GFP transgene labels skeletal progenitors in the periosteum.

Bone

Tournaire G,Stegen S,Giacomini G,Stockmans I,Moermans K,Carmeliet G,van Gastel N

12-0903-82 was used in Flow Cytometry to show that in adult mice, Nes-GFP cells are present in the periosteum and localize closely to blood vessels, but periosteal Nes-GFP cells express SSC and progenitor markers differently compared to Nes-GFP cells in the bone marrow.

Wed Apr 01 00:00:00 EDT 2020

Low-Dose Radiotherapy Ameliorates Advanced Arthritis in hTNF-α tg Mice by Particularly Positively Impacting on Bone Metabolism.

Frontiers in immunology

Deloch L,Derer A,Hueber AJ,Herrmann M,Schett GA,Wölfelschneider J,Hahn J,Rühle PF,Stillkrieg W,Fuchs J,Fietkau R,Frey B,Gaipl US

12-0903 was used in Flow cytometry/Cell sorting to study the underlying mechanisms by which low-dose radiotherapy mediates anti-inflammatory and pain-reducing effects in non-linear dose response relationships.

Mon Sep 30 00:00:00 EDT 2019

Rap1 deficiency-provoked paracrine dysfunction impairs immunosuppressive potency of mesenchymal stem cells in allograft rejection of heart transplantation.

Cell death & disease

Ding Y,Liang X,Zhang Y,Yi L,Shum HC,Chen Q,Chan BP,Fan H,Liu Z,Tergaonkar V,Qi Z,Tse HF,Lian Q

12-0903 was used in Flow cytometry/Cell sorting to indicate that Rap1 is essential to maintain the immunomodulatory function of MSCs.

Wed Mar 07 00:00:00 EST 2018

Low-Dose Radiotherapy Has No Harmful Effects on Key Cells of Healthy Non-Inflamed Joints.

International journal of molecular sciences

Deloch L,Rückert M,Fietkau R,Frey B,Gaipl US

12-0903 was used in Flow cytometry/Cell sorting to show that low-dose radiotherapy with particularly a single dose of 0.5 Gray has no harmful effects on cells of healthy joints.

Tue Oct 16 00:00:00 EDT 2018

Differential regulation of C5a receptor 1 in innate immune cells during the allergic asthma effector phase.

PloS one

Ender F,Wiese AV,Schmudde I,Sun J,Vollbrandt T,König P,Laumonnier Y,Köhl J

12-0903 was used in Flow cytometry/Cell sorting to demonstrate a complex regulation pattern of C5aR1 in the airways, lung tissue and lymph nodes of mice.

Thu Aug 17 00:00:00 EDT 2017

Mononuclear Phagocytes Are Dispensable for Cardiac Remodeling in Established Pressure-Overload Heart Failure.

PloS one

Patel B,Ismahil MA,Hamid T,Bansal SS,Prabhu SD

12-0903 was used in Flow cytometry/Cell sorting to assess for alterations in MPs.

Thu Aug 10 00:00:00 EDT 2017

A New Method to Stabilize C-Kit Expression in Reparative Cardiac Mesenchymal Cells.

Frontiers in cell and developmental biology

Wysoczynski M,Dassanayaka S,Zafir A,Ghafghazi S,Long BW,Noble C,DeMartino AM,Brittian KR,Bolli R,Jones SP

12-0903 was used in Flow cytometry/Cell sorting to investigate a new method for c-kit expressing cell isolation and stabilisation in reparative cardiac mesenchymal cells.

Thu Aug 18 00:00:00 EDT 2016

Multi-kilobase homozygous targeted gene replacement in human induced pluripotent stem cells.

Nucleic acids research

Byrne SM,Ortiz L,Mali P,Aach J,Church GM

12-0903 was used in Flow cytometry/Cell sorting to assess the efficiency of homologous recombination after trialling different targeting vector design parameters.

Wed Feb 18 00:00:00 EST 2015

Collaborative rewiring of the pluripotency network by chromatin and signalling modulating pathways.

Nature communications

Tran KA,Jackson SA,Olufs ZP,Zaidan NZ,Leng N,Kendziorski C,Roy S,Sridharan R

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 12-0903-82) in Flow Cytometry

Wed Feb 04 00:00:00 EST 2015

O-GlcNAcylation Negatively Regulates Cardiomyogenic Fate in Adult Mouse Cardiac Mesenchymal Stromal Cells.

PloS one

Zafir A,Bradley JA,Long BW,Muthusamy S,Li Q,Hill BG,Wysoczynski M,Prabhu SD,Bhatnagar A,Bolli R,Jones SP

12-0903 was used in Flow cytometry/Cell sorting to investigate how O-GlcNAcylation of intracellular proteins is involved in the regulation of mesenchymal stromal cell lineage commitment.

Wed Jun 29 00:00:00 EDT 2016

BMP-non-responsive Sca1+ CD73+ CD44+ mouse bone marrow derived osteoprogenitor cells respond to combination of VEGF and BMP-6 to display enhanced osteoblastic differentiation and ectopic bone formation.

PloS one

Madhu V,Li CJ,Dighe AS,Balian G,Cui Q

12-0903 was used in Flow cytometry/Cell sorting to study how combined action of VEGF and BMP on MSCs enhances osteoblastic differentiation of MSCs and increases their bone forming ability.

Fri Nov 20 00:00:00 EST 2015

Mouse Ly49G2+ NK cells dominate early responses during both immune reconstitution and activation independently of MHC.

Blood

Barao I,Alvarez M,Ames E,Orr MT,Stefanski HE,Blazar BR,Lanier LL,Anderson SK,Redelman D,Murphy WJ

12-0903 was used in Flow cytometry/Cell sorting to show that during reconstitution after HSCT and various activation stimuli, preferential expansion of the Ly49G2 receptor on NK cells occurs independently of MHC haplotype.

Thu Jun 30 00:00:00 EDT 2011

Hydrodynamic delivery of human IL-15 cDNA increases murine natural killer cell recovery after syngeneic bone marrow transplantation.

Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation

Barao I,Alvarez M,Redelman D,Weiss JM,Ortaldo JR,Wiltrout RH,Murphy WJ

12-0903 was used in Flow cytometry/Cell sorting to highlight cytokine gene transfer-based methods as a potential platform for natural killer cell recovery after bone marrow transplantation in mice.

Thu Dec 01 00:00:00 EST 2011

Characterization of fibroblasts responsible for cartilage destruction in arthritis.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Treese C,Mittag A,Lange F,Tarnok A,Loesche A,Emmrich F,Lehmann J,Sack U

12-0903 was used in Flow cytometry/Cell sorting to show that LS48 is an excellent tool to gain a deeper understanding of synovial fibroblasts in rheumatoid arthritis.

Tue Apr 01 00:00:00 EDT 2008

Hierarchy of Notch-Delta interactions promoting T cell lineage commitment and maturation.

The Journal of experimental medicine

Besseyrias V,Fiorini E,Strobl LJ,Zimber-Strobl U,Dumortier A,Koch U,Arcangeli ML,Ezine S,Macdonald HR,Radtke F

12-0903 was used in Flow cytometry/Cell sorting to study the Notch-Delta interactions that promote T cell development.

Mon Feb 19 00:00:00 EST 2007

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CD90.2 (Thy-1.2) Monoclonal Antibody (30-H12), PE, eBioscience™

CD90.2 (Thy-1.2) Antibody (12-0903-81) in Flow

Product Details

12-0903-81

Flow Cytometry (Flow)

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Performance Guarantee

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